NAD+ (Buffered)
A critical coenzyme studied in laboratory settings for cellular energy metabolism and aging research
About NAD+ (Buffered)
NAD+ (Nicotinamide Adenine Dinucleotide, CAS 53-84-9) is a critical coenzyme found in every living cell. This buffered formulation maintains stability for research applications studying cellular metabolism, DNA repair, and sirtuin activation pathways.
NAD+ was first discovered in 1906 by Arthur Harden and William John Young while studying fermentation in yeast extracts. They noticed that adding boiled yeast extract to unboiled extract dramatically accelerated fermentation—the heat-stable factor they identified would eventually be recognized as NAD+, one of the most important molecules in biochemistry.
The molecule's central role in metabolism became clear over the following decades. Hans von Euler-Chelpin received the 1929 Nobel Prize for elucidating NAD's structure and function. We now know NAD+ participates in over 500 enzymatic reactions, serving as an essential cofactor for enzymes that transfer electrons during cellular respiration and many other processes.
Interest in NAD+ surged in the early 2000s when researchers discovered its critical role in activating sirtuins—proteins linked to longevity in multiple organisms. The finding that NAD+ levels decline with age, and that boosting NAD+ could activate these longevity pathways, sparked intense research interest. This buffered formulation maintains pH stability for research applications.
Quick Facts
| CAS Number | 53-84-9 |
|---|---|
| Molecular Formula | C₂₁H₂₇N₇O₁₄P₂ |
| Molar Mass | 663.4 g/mol |
| Sequence | Dinucleotide (coenzyme) |
| Form | Lyophilized buffered powder |
| Purity | 99%+ (HPLC verified) |
| Third-Party Testing | Freedom Diagnostics (COA included) |
| Storage | Room temp 3–4 months; refrigerate post-reconstitution |
| Price (from) | $65.00 USD |
| SKU | PF-NAD-500MG |
How NAD+ (Buffered) Works
Research has examined NAD+'s mechanism as an essential coenzyme that serves as an electron carrier in redox reactions and as a substrate for NAD-consuming enzymes including sirtuins and PARPs.
Redox Reactions
NAD+ accepts electrons during glycolysis and the citric acid cycle, becoming NADH. This reduced form then donates electrons to the electron transport chain, driving ATP synthesis. The NAD+/NADH ratio is a key indicator of cellular metabolic state.
Sirtuin Activation
Sirtuins (SIRT1-7) are NAD+-dependent deacetylases that regulate metabolism, stress responses, and longevity. They consume NAD+ to remove acetyl groups from proteins, linking cellular energy status to gene expression and protein function.
PARP-Mediated DNA Repair
Poly(ADP-ribose) polymerases (PARPs) use NAD+ as a substrate to add ADP-ribose units to proteins at DNA damage sites. This modification recruits repair machinery and is essential for maintaining genomic integrity.
CD38 and NAD+ Consumption
CD38 is an NAD+-consuming ectoenzyme that increases with age and inflammation. Its activity contributes to age-related NAD+ decline, making CD38 a target of interest in aging research alongside NAD+ supplementation strategies.
Frequently Asked Questions
- What is NAD+ (Buffered)?
- NAD+ (Nicotinamide Adenine Dinucleotide, CAS 53-84-9) is a critical coenzyme found in every living cell. This buffered formulation maintains stability for research applications studying cellular metabolism, DNA repair, and sirtuin activation pathways.
- What is the CAS number for NAD+ (Buffered)?
- The CAS number for NAD+ (Buffered) is 53-84-9.
- What purity is Peptide Foundry's NAD+ (Buffered)?
- Peptide Foundry's research peptides are manufactured to 99%+ purity. Every batch is verified by an independent third-party laboratory (Freedom Diagnostics) using high-performance liquid chromatography (HPLC) for purity and identity, Limulus amebocyte lysate (LAL) assay for bacterial endotoxin, and inductively coupled plasma mass spectrometry (ICP-MS) for heavy-metal contamination. A Certificate of Analysis is published for every product and batch.
- How should NAD+ (Buffered) be stored?
- Lyophilized buffered NAD+ is best stored refrigerated (2-8°C) in its original sealed container; short periods at room temperature are acceptable if moisture is excluded. NAD+ is hygroscopic and oxidation-sensitive, so once reconstituted with bacteriostatic water, keep refrigerated, shielded from light, and use within 5-7 days for research requiring intact cofactor activity.
- Does NAD+ (Buffered) ship with a Certificate of Analysis?
- Yes. Every batch of every product ships with a Certificate of Analysis from Freedom Diagnostics, an independent third-party analytical laboratory. The COA documents HPLC purity, peptide identity, endotoxin testing, and heavy-metals screening.
- Is NAD+ (Buffered) sold for human use?
- No. All products sold by Peptide Foundry are strictly for in-vitro research and laboratory use only (RUO). They are not intended for human or animal consumption, therapeutic use, or diagnostic application.
Scientific References
- Verdin E. NAD+ in aging, metabolism, and neurodegeneration. Science. 2015;350(6265):1208-1213. PubMed: 26785480
- Imai S, Guarente L. NAD+ and sirtuins in aging and disease. Trends in Cell Biology. 2014;24(8):464-471. PubMed: 24786309
- Yoshino J, et al. NAD+ intermediates: The biology and therapeutic potential of NMN and NR. Cell Metabolism. 2018;27(3):513-528. PubMed: 29249689
This product is sold strictly for in-vitro research use only (RUO). It is not intended for human or animal consumption, therapeutic use, or diagnostic application.